Material and methods Naturally, mature ovum discharges some magnetic waves of different frequencies. When dam is discharged an ovum to produce female calf, the frequency of waves around the ovum repel y-chromosome bearing sperm that ovum can be fertilized only with X- chromosome bearing sperm. When the birth of male calf is desired, the second mechanism allows the ovum to produce waves which repel X-chromosome bearing sperm and get fertilize with Y-chromosome bearing sperm. The waves, which are responsible for the birth of male calf and female calf, were named as H-WAVES and M-WAVES respectively. These mechanisms make some special changes in follicular fluid around the ovum; for example, change in viscosity, pH, osmotic pressure or steriodogenic ratio that enable ovum to discharge H or M waves. This technology is equally effective in case of naturally mating or artificial insemination of the animals. After extensive experimentation, we have discover to mechanism named “RATAN PAK H (RPH)” AND “RATAN PAK M (RPM)” which are working to produce either male or female calf. RPH is responsible to charge follicular fluid in such a way that ovum produce H- waves, which repel X-chromosome bearing sperm presenting, birth of male calf. On the other hand, RPM manipulate ovum such a way that a female calf is produced.
Results Successful trials have been conducted in the area of Sargodha district of Punjab, Pakistan. Three species of animals like mare, cows and buffaloes were included in these trials until now, we have been worked on 732 animals the results obtained are very encouraging. The results of these experiments are present in Table 1
The trials are also being conduct now at different areas to confirm the results of our technology. WE know that this is a new approach for gender preselection and the idea is entirely different than that already existing one, therefore , it would not be accepted easily the scientist and the general public. However, we can say with confidence that we can produce at least 96% results with predetermined sex and we can prove it practically at any farm, or at any research institute. Discovery of such mechanism should be give a lot of new fields to scientists for their research and should be much more fruitful for farmers.

Table – 1: Number and species of animals have been experiment with Pak Shahpur Sex Choice Technology in Sargodha District
S. NO SPECIES OF ANIMALS NO.TREATED POSITIVE NEGATIVE POSITIVE%
1 COWS 462 460 02 99.56
2 BUFFLOES 126 123 03 97.61
3 MARES 12 11 01 91.96
TOTAL 732 726 06 96.27



Conclusion: Controlling gender through manipulation of ovum is an easy and reliable technology, which could be applied equally through natural mating or artificial insemination. Discovery of such mechanism should give a lot new fields to scientist for their research. This technology would have a significant impact on the genetics and economics of livestock production. Selection of gender through this technology could give farmers the choice of sex of offspring. Keeping in view all these advantages of general preselection offspring, it is now imperative to produce male or female calf at your own choice. Fortunately, it is now possible through Pak Shahpur choice sex (PSCS) Technology.